In Vitro Cytotoxicity Test

One of the most important indicators for biological evaluation in in vitro studies is cytotoxicity tests. In vitro cytotoxicity mechanisms are as follows:

- Chemicals such as pesticides destroy cell membranes,
- Inhibit protein synthesis,
- Irreversible binding to receptors

In In vitro cytotoxicity and cell viability assays are test methods used to detect cell death caused by the above-mentioned damages. When choosing cytotoxicity and cell viability test methods, some parameters are sought:

- Availability in the laboratory where the study will be performed,
- Test compounds,
- Detection mechanism,
- Specificity and sensitivity

In vitro cytotoxicity tests are performed to determine cell viability such as drug development or cancer research. In vitro cytotoxicity tests are classified according to the types of measurement:

- Dye exclusion: Trypan blue, eosin, Congo red, erythrosine B tests
- Colorimetric tests: MTT test, MTS test, XTT test, WST-1 test, WST-8 test, LDH test, SRB test, NRU test and crystal violet test
- Fluorometric tests: alamarBlue test and CFDA-AM test
- Luminometric tests